Novel Hepatitis E Virus in Ferrets, the Netherlands

Table 1. Primers used for amplification and sequencing of the ferret HEV genome* Primer Sequence, 5 3 Nucleotide position† FRHEV-F1 GGCTGGCGTTTGCTTGGAGG 256–275 FRHEV-R1 TTCGAATCCAACGCTGGTGAC 1158–1178 FRHEV-F2 GTACTATCACGGCCAATGAG 1077–1096 FRHEV-R2 CAGCCTATAGGGCATAGTAAG 1681–1701 FRHEV-F3 GCCCTGACCTTGGAGCTGAC 1592–1611 FRHEV-R3 CTATTGGCGGCGTTAACTAG 2078–2097 FRHEV-F4 GAGCTTTTGCCGGATGGGTC 2015–2034 FRHEV-R4 CACTAGCCCCTTATGGTCGA 2922–2941 FRHEV-F5 CTGTTGCCAAATCGGTCGTA 2865–2884 FRHEV-R5 GCATCAGACACGCCCAGCTC 3308–3327 FRHEV-F6 TGCCTTGACGCGCCACACTG 3250–369 FRHEV-PCR-InR CTCACCKGAATGCTTCTTCC 4272–4291 FRHEV-PCR-ExF TCCAGAAGGACTGCAACAAG 3876–3895 FRHEV-R7 CTCACCACGTCAGGAACAACC 4525–4545 FRHEV-F8 AGTTGAAGGTGGCGTTCCAC 4461–4480 FRHEV-R8 GCGCCTCGAGAATCGTAATC 5183–5202 FRHEV-F9 CGTGTTCCCGATGCCGGGCA 5114–5133 FRHEV-R9 CAGGCGAAGGCGTCGGCC 6164–6181 FRHEV3 RACE-ExF TATACCTCTGTTGAGGCTGC 6049–6068 FRHEV3 RACE-InF CCTTGGACTTTCGTCTGTTAC 6108–6128 FRHEV-5 end-F‡ GACCATCTAGCGACCTCCACTTTTTTTTTTTTTTTTTTTT NA FRHEV-5 end-R TGCATATCTCGACCGTCAGG 338–357 FRHEV-R10 TATTTGCTGGACCCCTTC 380–397 FRHEV-PCR-ExR CAAGATCACCGCCATGTTCCA 4319–4339 FRHEV-PCR-InF GTCAGGGCATWTCRGCSTGG 3933–3952 *HEV, hepatis E virus; FRHEV, ferret HEV; F, forward; R, reverse; RACE, rapid amplification of cDNA ends; NA, not applicable. †Positions of primers located in the complete genome are shown according to ferret HEV strains. ‡Nonviral primer.